Environmental Data: Gone-wild grapevines in forests host phytoplasma g...

Keywords:
FLAVESCENCE DORÉE
GENOTYPING
GRAPEVINE YELLOWS
ORIENTUS ISHIDAE
SCAPHOIDEUS TITANUS
SOUTHERN SWITZERLAND
SWITZERLAND
Keywords:
FLAVESCENCE DORÉE
GENOTYPING
GRAPEVINE YELLOWS
ORIENTUS ISHIDAE
SCAPHOIDEUS TITANUS
SOUTHERN SWITZERLAND
SWITZERLAND

Description

“Flavescence dorée” (FD) is a quarantine grapevine disease associated with FD phytoplasmas (FDp). In Switzerland, FD was identified in 2004 in the southern...

Citation

Oggier, A., Conedera, M., Debonneville, C., Schumpp, O., Rizzoli, A. (2024). Gone-wild grapevines in forests host phytoplasma genotypes linked to grapevines Flavescence doree epidemics in cultivated vineyards and competent vectors. EnviDat. https://www.doi.org/10.16904/envidat.503.

Resources

  • Online_Resource_1.docx

    Phytoplasma sequences from this work and reference strains with their GenBank accession number, hosts, geographic origin and reference.

    Online_Resource_1.docx

  • Table_1.docx

    Plot characteristics in terms of coordinates, region, mean slope, closest distance from nearest cultivated vineyard (DV), distance range between yellow sticky trap and nearest forest edge (DF), last time registered as cultivated vineyard (Last_T); absolute captures (N) and mean captures per hundred leaves (mean/100 leaves ± SE) during nymph collection for Scaphoideus titanus and Orientus ishidae, absolute captures (N) and mean captures per single yellow sticky trap (mean/trap ± SE).

    Table_1.docx

  • Table_2.docx

    Molecular characterization of the Flavescence dorée phytoplasma detected in Scaphoideus titanus and Orientus ishidae specimens collected on gone-wild grapevines; number of analyzed and infected insect specimens and genotype profile for the map, vmpA and malG genes.

    Table_2.docx

  • Table_3.docx

    Results of molecular analysis for the detection of Flavescence dorée phytoplasma in symptomatic and asymptomatic leaf samples collected from gone-wild grapevines; number of analyzed and infected leaf samples, and map genotype (conducted on all FDp-infected samples).

    Table_3.docx

  • Fig1.tif

    Location of the experimental plots in Canton Ticino (a) Location of the study area within Switzerland; T = Ticino (b) Black dots: plots hosting GWGVs monitored for the presence of FDp-infected insect and plant samples. Sopra = Sopraceneri (Ticino); Sotto = Sottoceneri (Ticino). Grey areas = cultivated vineyard area.

    Fig1.tif

  • Fig2a.tif

    Flavescence dorée detection on the analyzed insect (a) and gone-wild grapevine (b) samples (a) Circles: location with insect vectors tested for Flavescence dorée phytoplasma; horizontal bar: S. titanus FDp-infected with map-genotype M54; diagonal line top right to bottom left; O. ishidae FDp-infected with map-genotype M54; diagonal line top left to bottom right: O. ishidae FDp-infected with map-genotype M50 (b) Circles: Gone-wild grapevines (GWGV) tested for FDp; vertical bar: symptomatic and FDp-infected samples; horizontal bar: asymptomatic and FDp-infected samples. Sopra = Sopraceneri (Ticino); Sotto = Sottoceneri (Ticino). Grey areas = cultivated vineyard area.

    Fig2a.tif

  • Fig3.jpg

    Phylogenetic tree of the map (a; partial) and vmpA (b) genes sequences from Scaphoideus titanus, Orientus ishidae and gone-wild grapevines obtained in this work and reference strains from Genbank (see Online Resource 1). Maximum likelihood phylogeny based on nucleotide sequences of (A) map (543 bp) and (B) vmpA (465 bp) genes. The numbers on branches indicate the level of bootstrap support (500 replicates). Support values above 70% are labeled. The scale bar shows the number of substitutions per site.

    Fig3.jpg